Microsoft and Google came second and third respectively, but CRH topped the poll with a turnover of €18.6 billion last year.
The poll ranks the top 1,000 companies in Ireland by turnover.
Irish-owned businesses to appear in the top 20 were Paddy Power, Dunnes Stores, Ryanair, ESB, Kerry Group, and Musgrave.
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Microsoft had a turnover of close to €14 billion, with Google at €12.5 billion, although social gaming company Zynga was the “best performer” in this sector.
The poll is available online at www.top1000.ie.