The Coombe Hospital in Dublin was hit by a cyber-attack overnight on Wednesday.
However it says services are continuing as normal.
Management say it has locked down all its IT systems "on a precautionary basis".
The hospital says it is working closely with the Health Service Executive (HSE) to resolve this matter.
The HSE says there is no evidence of any impact outside the Coombe Hospital, but a broader examination is underway.
The Coombe systems have also been disconnected from the National Health Network.
It comes almost seven month after a widespread cyber-attack against the HSE.
HSE CEO Paul Reid described the ransomware attack in May as "quite sophisticated and very targeted".
While data of around 520 patients emerged online later that month, as well as some corporate documents.
The data included sensitive patient data, minutes of meetings and correspondence with patients.