Fianna Fáil's Lisa Chambers has lost her seat in Mayo overnight.
The party's Brexit spokesperson had served as a TD for the constituency since 2016.
The final seat was taken by Fine Gael's candidate Alan Dillon.
Mr Dillon, a former Mayo footballer, replaced former party leader Enda Kenny - who did not run for re-election.
In a tweet after the result, he said: "It's been quite a 24 hours. Thank you to the Mayo electorate for electing me to the 33rd Dáil.
"It's an honour & a privilege".
It’s been quite a 24 hours. Thank you to the #Mayo electorate for electing me to the 33rd Dáil. It’s an honour & a privilege. I want to congratulate @MichaelRingFG & @daracalleary on their re-election and @conwaywalsh on her election. Also to the count staff, thank you. #GE20202 pic.twitter.com/pnqDVs62nF
— Alan Dillon (@Alan_Dillon) February 10, 2020
Dara Calleary of Fianna Fáil was also elected on the last count.
Fine Gael's Michael Ring and Rose Conway-Walsh of Sinn Féin were both elected on the first count.
Ms Chambers came under scrutiny after she cast votes in the Dáil on behalf of a colleague late last year.
She admitted recording a vote on behalf of Mr Calleary.
She said she recorded the vote by mistake after sitting in the wrong seat.
She then moved to her own seat, voted again and did not correct the record.
She - along with Niall Collins and Timmy Dooley - apologised for their roles in the scandal.
Deputy Chambers said: "The vote was lost by a large number, so I did not inform the teller at the time of the vote cast in error as I genuinely thought it was an insignificant error.
"I fully accept now that I should have done so at the time, and I sincerely apologise to this House and to the public for not doing so".