Static speed cameras are set to go live in County Mayo and County Galway tomorrow.
The cameras will be operational along the N17 in Mayo and on the N59 in Galway.
Static speed cameras are set up in one permanent location and they measure the speed of passing cars at that particular point.
Anyone caught by the cameras to be driving over the 100km/h will be automatically subject to prosecution, Gardaí have said.
The penalty for speeding is a Fixed Charge Notice of €160 and three penalty points.
In May, Gardaí said they are aiming to have nine fully functional static speed cameras on Irish roads by the end of the year.
Other proposed locations for static speed cameras include the N25 in Waterford, the R772 in Wicklow, the N14 in Donegal, the N80 in Carlow, Dolphin's Barn in Dublin, the N22 in Cork and the N69 in Limerick.
These areas were identified as locations with a high level of fatal and serious injuries.
Feedback from stakeholders also played a role in selecting these locations.
Research from the Institute of Transport Economics, Norway suggests that static speed cameras can lead to drivers reducing their speed, which is a major factor in road deaths.
When the initiative was announced, Roads Policing and Community Engagement Assistant Commissioner Paula Hilman described these cameras as "highly effective".
"Speed cameras slow drivers down," Ms Hilman said.
"The lower speeds people drive at, the lower the number of road deaths."
Gardaí have said they will provide further information on additional static speed cameras as the rollout continues.
Feature image shows a static speed camera, Alamy.