Assistant coach William Servat is the latest member of France's management to test positive for COVID-19.
Head coach Fabien Galthie and another unnamed person returned positive results on Tuesday so all three will now have to isolate for seven days.
The French Rugby Federation said in a statement:
"Following additional PCR tests of the entire France group carried out on the evening of Tuesday February 16, all the players tested negative, which confirms the result of Monday February 15.
"They will be able to return to their homes today and are instructed to maintain their isolation.
"A member of management, William Servat, has tested positive for COVID-19. This brings the number to three cases diagnosed positive for COVID-19 within the framework. No symptoms have been observed to date.
"Additional PCR tests will be carried out at home on Friday February 19, as well as Sunday February 20 in the evening, upon arrival of the players and the management at the National Rugby Center in Marcoussis.
"Once the tests have been carried out, everyone will be placed in isolation pending the results.
"The players all tested negative on Monday and Tuesday and will self-isolate at home for the rest of the week before being tested at home on Friday and again on Sunday when the squad gathers again."
France have started the Six Nations with wins over Italy and Ireland and will be back in action on February 28th against Scotland.
The IRFU said on Tuesday that there were no positive results when the players, management and members of the backroom team were tested on Monday.
Team of Us. Everyone In.
Vodafone. The main sponsor of the Irish Rugby Team