Scotland's Stuart Armstrong, Kieran Tierney and Ryan Christie will all miss their Euro 2020 play-off with Israel after Armstrong tested positive for coronavirus.
Tierney and Christie returned negative tests, but will have to self-isolate as "close contacts" of the Southampton midfielder.
The trio will also sit out their upcoming Nations League matches against Slovakia and the Czech Republic. Tierney will miss Arsenal's clash with Manchester City after the international break while Christie won't be available for Celtic's Old Firm derby with Rangers.
“We were made aware of the positive test this morning and I reiterate Steve’s view that the health and safety of those involved is paramount," Scotland FA chief executive Ian Maxwell said in a statement.
"We will maintain constant dialogue with Dr John MacLean to ensure that those who are required to self-isolate are given the full support of the Scottish FA in conjunction with their clubs.
“Equally, the protocols and procedures in place are designed to ensure that the remaining members of the squad – who have all returned two rounds of negative tests – can continue to prepare for the forthcoming matches in a safe and secure environment.”
"While this is obviously disappointing news the most important thing is the health and safety of the individuals involved and the wider group," Scotland manager Steve Clarke added.
"We have informed the respective clubs from whom we have borrowed the players and backroom staff and we now have to prepare for a huge match ahead tomorrow."
